Rat Serum Contains a Developmentally Regulated Cholinergic Inducing Activity
- Creators
- Wolinsky, Eve J.
- Patterson, Paul H.
Abstract
Sympathetic neurons cultured in defined medium do not develop the ability to produce acetylcholine, as do neurons grown with serum supplementation (lacovitti, L., M. I. Johnson, T. H. Joh, and R. P. Bunge (1982) Neuroscience 7:2225–2239; Wolinsky, E. J., S. C. Landis, and P. H. Patterson (1985) J. Neurosci. 5: 1497–1508). The implication that rat serum contains cholinergic inducing activity is further explored here. Dependence of cholinergic induction on serum concentration is demonstrated, and the activity is shown to reside in a macromolecular fraction. Very little cholinergic inducing activity is present in serum obtained from animals younger than 9 postnatal days. This age dependence correlates with the time of transition from noradrenergic to cholinergic transmitter status by the sympathetic innervation of the rat sweat gland in vivo (Landis, S. C., and D. Keefe (1983) Dev. Biol. 98: 349–372).
Additional Information
© 1985 Society for Neuroscience. For the first six months after publication SfN's license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received July 2, 1984; Revised October 5, 1984; Accepted October 8, 1984. This work was supported by grants from the National Institute of Neurological and Communicative Disorders and Stroke. E. J. W. was a predoctoral trainee of the National Institute of General Medical Sciences. P. H. P. was a Rita Allen Foundation Fellow and a McKnight Foundation Neuroscience Development Awardee. Support was also provided by grants from the National Institute of Neurological and Communicative Disorders and Stroke to P. H. P. We would like to thank Doreen McDowell for assistance with the cell culturing. We also thank Ors. S. C. Landis and T. M. Jessell for helpful discussions of the manuscript.Attached Files
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Additional details
- PMCID
- PMC6565265
- Eprint ID
- 67150
- Resolver ID
- CaltechAUTHORS:20160517-082439647
- National Institute of Neurological and Communicative Disorders and Stroke (NINCDS)
- National Institute of General Medical Sciences
- Rita Allen Foundation
- McKnight Foundation
- NIH
- Created
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2016-05-17Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field