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Published July 1982 | public
Journal Article

Interhelical DNA-DNA crosslinking. Bis(monoazidomethidium)octaoxahexacosanediamine: a probe of packaged nucleic acid

Abstract

We describe the synthesis and purification of a new light-sensitive bifunctional molecule, bis(monoazidomethidium)octaoxahexacosanediamine (BAMO) (1), which contains all the criteria presumed necessary for an interhelical DNA-DNA cross-linker: a photoreactive DNA intercalator, monoazidomethidium, and a polyethyleneoxy chain sufficiently long (>25 Å) to span contiguous nucleic acid double helices. We also present evidence that BAMO efficiently cross-links packaged nucleic acid in intact bacteriophage λ.

Additional Information

© 1982 American Chemical Society. Received March 10, 1982. We are grateful to the National Institutes of Health (GM 22909) for generous support and to Professor Charles Cantor, Columbia University, for helpful discussions. Contribution No. 6584 from the Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, California 91125.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023