Published November 1, 1988
| public
Journal Article
Double strand cleavage of genomic DNA at a single site by triple helix formation
Abstract
The sequence-specific cleavage of double helical DNA by restriction endonucleases is essential for many techniques in molecular biology, including gene isolation, DNA sequencing, and recombinant DNA manipulations. With the advent of pulsed-field gel electrophoresis, the separation of large segments of DNA is now possible. However, the recognition sequences of naturally occurring restriction enzymes are in the range of 4-8 base pairs, and hence their sequence specificites may be inadequate for isolating genes from large chromosomes (10^8 base pairs in size) or mapping genomic DNA.
Additional Information
© 1988 American Chemical Society. Received July 22, 1988. Publication Date: November 1988. This work was supported in part by the National Institutes of Health (GM 35724), the Caltech Consortium in Chemistry and Chemical Engineering (Founding members: E. I. duPont de Nemours and Company, Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation), and a National Research Service Award (T32GM07616) from the NIH-GM to S.A.S.Additional details
- Eprint ID
- 67001
- Resolver ID
- CaltechAUTHORS:20160511-135304681
- GM-35724
- NIH
- Caltech Consortium in Chemistry and Chemical Engineering
- T32GM07616
- NIH Predoctoral Fellowship
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2021-11-11Created from EPrint's last_modified field