Published June 11, 1992
| Published
Journal Article
Open
The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving
- Creators
- Beal, Peter A.
-
Dervan, Peter B.
Abstract
The influence of sixteen base triplet changes at a single position within a pur·pur·pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G·GC, A·AT and T·AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25°C). Weaker interactions were detected for the C·AT, A·GC and T·CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.
Additional Information
© 1992 Oxford University Press. Received February 20, 1992; Revised and Accepted May 5, 1992. We are grateful for generous support from the National Institutes of Health (HG00329) and a DOE predoctoral fellowship for P.A.B.Attached Files
Published - Nucl._Acids_Res.-1992-Beal-2773-6.pdf
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Nucl._Acids_Res.-1992-Beal-2773-6.pdf
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Additional details
- PMCID
- PMC336921
- Eprint ID
- 66944
- Resolver ID
- CaltechAUTHORS:20160510-142116893
- HG00329
- NIH
- Department of Energy (DOE)
- Created
-
2016-05-19Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field