Published June 2, 1993
| public
Journal Article
Cooperative oligonucleotide-directed triple helix formation at adjacent DNA sites
Chicago
An error occurred while generating the citation.
Abstract
The changes in binding free energies due to cooperativity for oligodeoxyribonucleotides bound to adjacent sites by triple helix formation have been determined by quantitative affinity cleavage titrations. A 20-fold enhancement in equilibrium association constant is realized for an l lmer pyrimidine oligonucleotide binding in the presence of a neighboring bound site at 24 °C and pH 7.0 (25 mM Tris-OAc, 10 mM NaCl, 1 mM spermine). This corresponds to an increase in binding free energy of 1.8 ± 0.2 kcal mol^(-1). This cooperativity is not observed when the two binding sites are separated by one base pair.
Additional Information
© 1993 American Chemical Society. Received January 25, 1993. This work was supported by the National Institute of Health (Grant HG 00329) and a Damon Runyon-Walter Winchell Cancer Research Fund postdoctoral fellowship for M.D.D. We thank Scott F. Singleton for helpful discussions.Additional details
- Eprint ID
- 66924
- Resolver ID
- CaltechAUTHORS:20160510-135312755
- NIH
- HG00329
- Damon Runyon-Walter Winchell Cancer Research Fund
- Created
-
2016-05-19Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field