Published May 10, 1994
| public
Journal Article
Phosphorothioate oligonucleotide-directed triple helix formation
Chicago
Abstract
Phosphorothioate oligodeoxyribonucleotides were tested for their ability to recognize double-helical DNA in two distinct triple helix motifs. Purine-rich oligonucleotides containing a diastereomeric mixture of phosphorothioate or stereoregular (all RP) phosphorothioate linkages are shown to form triple-helical complexes with affinities similar to those of the corresponding natural phosphodiester oligonucleotides. In contrast, pyrimidine-rich phosphorothioate oligonucleotides containing a mixture of diastereomeric or stereoregular (all RP) linkages do not bind to double-helical DNA with measurable affinity. These observations have implications for triple helix structure and for biological applications.
Additional Information
© 1994 American Chemical Society. Received February 4, 1994; Revised Manuscript Received March 7, 1994. Abstract published in Advance ACS Abstracts, April 15, 1994. Supported by a predoctoral fellowship from the Howard Hughes Medical Institute (to J.G.H.) and by research grants from the NIH (AR40780 to B.J.W. and GM35724 to P.B.D.).Additional details
- Eprint ID
- 66918
- DOI
- 10.1021/bi00184a002
- Resolver ID
- CaltechAUTHORS:20160510-133746868
- Howard Hughes Medical Institute (HHMI)
- NIH
- AR40780
- NIH
- GM-35724
- Created
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2016-05-18Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field