Published July 11, 1994
| Published
Journal Article
Open
Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation
- Creators
- Staubli, Andrea B.
-
Dervan, Peter B.
Chicago
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Abstract
The non-natural pyrido[2,3-d]pyrimidine nucleoside F, which pairs preferentially with guanine (G) and adenine (A) within double-helical DNA, recognizes with high selectivity AT base pairs within triple-helical complexes. These observations suggest that F may exist in different tautomeric forms within double-helical and triple-helical complexes. Analysis of the base stacking properties of this extended ring system using two oligodeoxyribonucleotides containing terminal thymines and/or pyrido[2,3-d]pyrimidines bound to adjacent sites showed a decrease in free energy of binding in a triple-helical complex in the order (5′ – 3′) TT > FT > TF > FF.
Additional Information
© 1994 Oxford University Press. Received March 4, 1994; Revised and Accepted May 17, 1994. Plasmids pPG1133 and pMD5152 were obtained from Justine Garvey. We are thankful for the support from the Office of Naval Research and for a postdoctoral fellowship from the Swiss National Science Foundation to A.B.S.Attached Files
Published - Nucl._Acids_Res.-1994-Staubli-2637-42.pdf
Files
Nucl._Acids_Res.-1994-Staubli-2637-42.pdf
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Additional details
- PMCID
- PMC308221
- Eprint ID
- 66910
- Resolver ID
- CaltechAUTHORS:20160510-132907922
- Office of Naval Research (ONR)
- Swiss National Science Foundation (SNSF)
- Created
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2016-05-19Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field