Published April 1998
| public
Journal Article
Structure of a stereoregular phosphorothioate DNA/RNA duplex
Chicago
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Abstract
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-R_p)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
Additional Information
© 1998 Nature Publishing Group. Received 17 October 1997; accepted 30 January 1998. We are grateful to the NIH for research support and the HHMI for a predoctoral fellowship to J. G. H.Additional details
- Eprint ID
- 66867
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- CaltechAUTHORS:20160510-094622267
- NIH
- Howard Hughes Medical Institute (HHMI)
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