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Published April 1998 | public
Journal Article

Structure of a stereoregular phosphorothioate DNA/RNA duplex

Abstract

In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-R_p)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.

Additional Information

© 1998 Nature Publishing Group. Received 17 October 1997; accepted 30 January 1998. We are grateful to the NIH for research support and the HHMI for a predoctoral fellowship to J. G. H.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023