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Published December 1978 | public
Journal Article

The single-copy DNA sequence polymorphism of the sea urchin strongylocentrotus purpuratus

Abstract

The single-copy DNA sequence difference between individual sea urchins of the species Strongylocentrotus purpuratus has been estimated by comparing the thermal stability of reassociated DNA duplexes from two individuals with that for DNA from an individual. Thermal stability was measured by hydroxyapatite thermal chromatography, S1 nuclease resistance after heating in a solvent which neutralizes the effect of DNA base composition, and spectrophotometric melting. One pair of individuals appear to differ from each other in about 4% of the nucleotide pairs of their single-copy DNA sequence. The differences in DNA sequence among individuals in local populations are not distinguishably smaller than those among populations as far apart as 2000 kilometers along the Pacific coast of North America.

Additional Information

© 1978 MIT. Received June 19, 1978; revised July 28, 1978. We acknowledge the excellent technical assistance of Richard Rohan. This work has been supported by a grant from the National Institute of General Medical Sciences. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023