Introduction of Cloned DNA into Sea Urchin Egg Cytoplasm: Replication and Persistence during Embryogenesis
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1.
California Institute of Technology
Abstract
Cloned DNA sequences were introduced into the cytoplasm of unfertilized sea urchin eggs by a simple microinjection technique. Sperm was then added, and development allowed to proceed. If linearized plasmids are injected they form random concatenates, and during the early development of the embryos replicate repeatedly. Eukaryotic sequences are not required for replication of the exogenous DNA. Injected supercoiled DNAs neither ligate nor replicate. Both forms of exogenous DNA persist in the embryo through pluteus stage.
Copyright and License
© 1985 Academic Press, Inc.
Acknowledgement
We thank Frances Teng for expert and intelligent technical assistance. We are grateful to Anita Colin for advice on microinjection procedures, and to Professors Norman Davidson, Barbara Wold, and Ellen Rothenberg for helpful and critical reviews of the manuscript. This research was supported by NIH Grant HD-05753. A.P.M. and C.N.F. were supported by American Cancer Society, California Division fellowships (J-33-82 and S-11-83, respectively) and K.S.K. by an NIH postdoctoral training grant (HD-07257).
Additional details
- Eprint ID
- 66145
- Resolver ID
- CaltechAUTHORS:20160414-080122940
- NIH
- HD-05753
- American Cancer Society, California Division
- J-33-82
- American Cancer Society, California Division
- S-11-83
- NIH Postdoctoral Fellowship
- HD-07257
- Created
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2016-04-14Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering (BBE)