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Published April 1997 | public
Journal Article

Complete Sequence of SpHox8 and Its Linkage in the Single Hox Gene Cluster of Strongylocentrotus purpuratus

Abstract

SpHox8 is the paralog group 8 Hox gene of Strongylocentrotus purpuratus. This identification follows from an analysis of the sequence of the complete open reading frame of a late-gastrula-stage cDNA clone; from direct linkage to adjacent Hox genes in a fosmid contig; and from blot hybridizations carried out on pulse field gel electrophoretic separations. SpHox8 is a single-copy gene, and there is only one Hox gene cluster per genome in S. purpuratus.

Additional Information

© 1997 Springer-Verlag New York Inc. Received: 7 November 1996. Accepted: 4 December 1996. It is a pleasure to acknowledge the assistance of Dr. Tom Wilkie in the design of oligonucleotides and of Valeria Mancino with pulse field gel electrophoesis. We are grateful to Drs. Kevin Peterson and Roy Britten of this Institute for reviews of drafts of the manuscript. This work was supported by NICHHD grant 05753 and by the Stowers Institute for Medical Research.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023