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Published June 1993 | public
Journal Article

Distribution of CD9 in the Developing and Mature Rat Nervous System

Abstract

CD9 is a cell surface protein implicated in intercellular signaling that has been identified in selected cell types of the hematopoietic system. To begin a study of the role of CD9 in the developing and adult nervous system, we used the anti-rat CD9 monoclonal antibody ROCA2 to determine the distribution of this protein. The identity of the antigen in these tissues was confirmed by immunoblotting and peptide sequencing. Early embryonic sympathetic and dorsal root ganglion sensory neurons and adrenal chromaffin cells all express CD9. ROCA2 also labels the somas, axons, and growth cones of cultured sympathetic and sensory neurons. In the central nervous system (CNS), CD9 is transiently and specifically expressed in embryonic spinal motorneurons. In the adult, central and peripheral glia intensely express CD9. Thus, CD9 is developmentlly regulated in a variety of peripheral and central neurons and glia, including proliferating progenitors as well as mature cells. These findings suggest that CD9 may have diverse roles in the nervous system.

Additional Information

© 1993 Wiley-Liss, Inc. Received March 4, 1993. We thank Li-Ching Lo and Dr. David Anderson for the mAb 192IgG, Dr. Linda Parysek for the anti-peripherin antiserum, Dr. Zaven Kaprielian for suggestions and for his help with the biochemistry, and David Anderson, Lisa Banner, Arlene Chiu, Michael Hadjiargyrou, and Zaven Kaprielian for comments on the manuscript. This project was supported by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust to P.H.P., and an Evelyn Sharp fellowship, a Helen G. and Arthur McCallum fellowship, and a grant from the Amyotrophic Lateral Sclerosis Association (ALSA) to S.T.

Additional details

Created:
August 20, 2023
Modified:
October 18, 2023