Morphological Domains of Lewis-X/FORSE-1 Immunolabeling in the Embryonic Neural Tube Are Due to Developmental Regulation of Cell Surface Carbohydrate Expression
Abstract
The Lewis-X (LeX) carbohydrate epitope, recognized by the FORSE-1 monoclonal antibody (mAb), shares expression boundaries with neural regulatory genes and may be involved in patterning the neural tube by creating domains of differential cell adhesion. The present experiments focus on the question of what determines the expression pattern of LeX in embryonic rat brain. Comparisons of FORSE-1-positive glycolipid and protein antigens in embryonic, early postnatal, and adult tissues show that the LeX epitope is carried primarily by glycolipids during embryonic development and by a proteoglycan and glycoproteins in postnatal and adult tissue. Immunohistochemistry using FORSE-1 and an antibody to the proteoglycan phosphacan, which carries LeX, shows that the distribution of LeX is more restricted than phosphacan. These observations suggest that the precise spatial regulation of FORSE-1 binding in the embryonic forebrain is due to the expression pattern of the LeX carbohydrate on glycolipids, rather than to the transcriptional regulation of a carrier protein.
Additional Information
© 1999 Academic Press. Received for publication September 1, 1998. Revised April 20, 1999. Accepted April 20, 1999. We thank Drs. Renee and Richard Margolis for the anti phosphacan Ab, Dr. Andrea Streit for the L5 mAb and Dr. Jean-Francois Bouhours for the antibody to a(1,3)galactose. We thank Drs. Jane Dodd and Carol Mason for providing lab facilities in which some of these experiments were completed. We thank Dr. Janet Baer for the pig kidney sample. We thank Drs. John Lowe, John Magnani, Juergen Mai, and Carol Mason for informative discussions and Drs. Lynda Erskine, Riva Marcus, Carol Mason, Andrea Streit, and Shubha Tole for carefully reading and commenting on the manuscript. This research was funded by a Helen Hay Whitney Fellowship to K.L.A., a Caltech Summer Undergraduate Research Fellowship to A.D., Grant P01 NS30532, Project 2, covering the work of G.A.M. in the lab of Dr. Carol Mason, and an NINDS grant to P.H.P.Additional details
- Eprint ID
- 65604
- DOI
- 10.1006/dbio.1999.9308
- Resolver ID
- CaltechAUTHORS:20160323-075527757
- Helen Hay Whitney Fellowship
- P01 NS30532
- NIH
- Caltech Summer Undergraduate Research Fellowship (SURF)
- Created
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2016-03-23Created from EPrint's datestamp field
- Updated
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2023-06-06Created from EPrint's last_modified field