Published April 2016
| Supplemental Material + Accepted Version
Journal Article
Open
A new view into prokaryotic cell biology from electron cryotomography
- Creators
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Oikonomou, Catherine M.
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Jensen, Grant J.
Abstract
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to 'macromolecular' (~4 nm) resolution, revealing the basic architectures of complete nanomachines and their arrangements in situ. Since its inception, ECT has advanced our understanding of many aspects of prokaryotic cell biology, from morphogenesis to subcellular compartmentalization and from metabolism to complex interspecies interactions. In this Review, we highlight how ECT has provided structural and mechanistic insights into the physiology of bacteria and archaea and discuss prospects for the future.
Additional Information
© 2016 Macmillan Publishers Limited. Published online 29 February 2016. The authors apologize that they could not discuss all of the work in this burgeoning field. The authors thank members of the Jensen laboratory for helpful comments on the manuscript, and J. Ding and Y.-W. Chang for producing the accompanying movie. The authors also thank L. Sockett (University of Nottingham) for the gift of the Bdellovibrio bacteriovorus strain imaged in figure 1 and shown in the accompanying movie. Microbial electron cryotomography (ECT) in the Jensen laboatory is supported, in part, by the Howard Hughes Medical Institute, the US National Institutes of Health (grants RO1 GM101425 and RO1 GM094800), the Beckman Institute at Caltech, Caltech's Center for Environmental Microbial Interactions, and gifts to Caltech from the Gordon and Betty Moore Foundation and the Agouron Institute. The authors declare no competing interest.Errata
It has recently come to the authors' attention that this Review is the first place in which Figure 1 was published. Yi-Wei Chang, who collected the data for Figure 1 and helped to revise the text, should have been included as an author. Therefore, on the title page, the corrected author list should read Catherine M. Oikonomou, Yi-Wei Chang and Grant J. Jensen.Attached Files
Accepted Version - nihms882786.pdf
Supplemental Material - nrmicro.2016.7-s1.mov
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nihms882786.pdf
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Additional details
- PMCID
- PMC5551487
- Eprint ID
- 65102
- Resolver ID
- CaltechAUTHORS:20160307-090319472
- Howard Hughes Medical Institute (HHMI)
- RO1 GM101425
- NIH
- RO1 GM094800
- NIH
- Caltech Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- Caltech Center for Environmental Microbial Interactions (CEMI)
- Created
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2016-03-08Created from EPrint's datestamp field
- Updated
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2023-06-01Created from EPrint's last_modified field
- Caltech groups
- Caltech Center for Environmental Microbial Interactions (CEMI)