Published March 12, 2008
| Accepted Version + Supplemental Material
Journal Article
Open
Electrical Detection of TATA Binding Protein at DNA-Modified Microelectrodes
Chicago
Abstract
A simple method for the electrochemical detection of TATA-binding protein is demonstrated at DNA-modified microelectrodes. The assay is general and based on the interruption of DNA-mediated charge transport to Nile Blue, a redox-active probe covalently attached to the DNA base pair stack. Nanomolar quantities of TATA binding protein can be detected on the microelectrodes even in the presence of micromolar amounts of bovine serum albumin, EndonucleaseIII, or Bam HI methyltransferase. The scheme outlined provides a basis for the sensitive electrical detection of numerous proteins on a single DNA chip.
Additional Information
© 2008 American Chemical Society. Received November 28, 2007. Publication Date (Web): February 14, 2008. We are grateful to the NIH (GM61077) for their financial support.Attached Files
Accepted Version - nihms97829.pdf
Supplemental Material - ja7106756_si_002.pdf
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ja7106756_si_002.pdf
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Additional details
- PMCID
- PMC2747583
- Eprint ID
- 64812
- DOI
- 10.1021/ja7106756
- Resolver ID
- CaltechAUTHORS:20160226-135927612
- NIH
- GM61077
- Created
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2016-02-29Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field