Analyzing the relationship between decorrelation time and tissue thickness in acute rat brain slices using multispeckle diffusing wave spectroscopy
- Creators
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Brake, Joshua
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Jang, Mooseok
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Yang, Changhuei
Abstract
Novel techniques in the field of wavefront shaping have enabled light to be focused deep inside or through scattering media such as biological tissue. However, most of these demonstrations have been limited to thin, static samples since these techniques are very sensitive to changes in the arrangement of the scatterers within. As the samples of interest get thicker, the influence of the dynamic nature of the sample becomes even more pronounced and the window of time in which the wavefront solutions remain valid shrinks further. In this paper, we examine the time scales upon which this decorrelation happens in acute rat brain slices via multispeckle diffusing wave spectroscopy and investigate the relationship between this decorrelation time and the thickness of the sample using diffusing wave spectroscopy theory and Monte Carlo photon transport simulation.
Additional Information
© 2016 Optical Society of America. Received 6 October 2015; revised 4 December 2015; accepted 26 December 2015; posted 5 January 2016 (Doc. ID 251465); published 1 February 2016. Funding: National Institute of Biomedical Imaging and Bioengineering (NIBIB) (1F31EB021153-01); National Institute of Neurological Disorders and Stroke (NINDS) (1U01NS090577-01); National Institutes of Health (NIH) (1DP2OD007307-01); Donna and Benjamin M. Rosen Bioengineering Center; GIST-Caltech (CG2012). J. B. acknowledges support from an NIH NRSA Predoctoral Fellowship and the Donna and Benjamin M. Rosen Bioengineering Center. The authors would also like to thank Dr. Cheng Xiao for his help in preparing the brain tissue slices.Attached Files
Published - josaa-33-2-270.pdf
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Additional details
- PMCID
- PMC4783160
- Eprint ID
- 64563
- Resolver ID
- CaltechAUTHORS:20160218-123702274
- NIH Postdoctoral Fellowship
- 1F31EB021153-01
- NIH
- 1U01NS090577-01
- NIH
- 1DP2OD007307-01
- Donna and Benjamin M. Rosen Bioengineering Center
- GIST-Caltech Research Collaboration
- CG2012
- NIH Predoctoral Fellowship
- National Institute of Neurological Disorders and Stroke (NINDS)
- National Institute of Biomedical Imaging and Bioengineering
- Created
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2016-02-18Created from EPrint's datestamp field
- Updated
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2022-05-12Created from EPrint's last_modified field
- Caltech groups
- Rosen Bioengineering Center