FGF Signaling Mediates Regeneration of the Differentiating Cerebellum through Repatterning of the Anterior Hindbrain and Reinitiation of Neuronal Migration
- Creators
- Köster, Reinhard W.
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Fraser, Scott E.
Abstract
To address the regenerative capability of the differentiating hindbrain, we ablated the cerebellum in wild-type and transgenic zebrafish embryos. These larvae showed no obvious locomotive malfunction several days after the ablation. Expression analysis and in vivo time-lapse recording in GFP (green fluorescent protein)-transgenic embryos indicate that cerebellar neuronal cells can regenerate from the remaining anterior hindbrain. The onset of regeneration is accompanied by repatterning within the anterior hindbrain. Inhibition of FGF signaling immediately after cerebellar ablation results in the lack of regenerating cerebellar neuronal cells and the absence of cerebellar structures several days later. Moreover, impaired FGF signaling inhibits the repatterning of the anterior hindbrain and the reexpression of rhombic lip marker genes soon after cerebellar ablation. This demonstrates that the hindbrain is highly plastic in recapitulating early embryonic differentiation mechanisms during regeneration. Moreover, the regenerating system offers a means to uncouple cerebellar differentiation from complex morphogenetic tissue rearrangements.
Additional Information
© 2006 Society for Neuroscience. For the first six months after publication SfN's license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Jan. 10, 2006; revised May 29, 2006; accepted May 31, 2006. This work was generously supported by the National Institutes of Health, the Caltech Biological Imaging Center, and the German Ministry for Education and Research through BioFuture Award 0311889. We thank Tanya Demyanenko and Aura Keeter for excellent technical assistance and animal care. We thank Nilima Prakash for critical discussion of this manuscript. We thank the members of the Fraser, Wurst, and Bally-Cuif laboratories for discussion and helpful suggestions. We are grateful to Shuo Lin for providing us with the transgenic gata1:gfp zebrafish line. For the generous gift of the zebrinII antibody, we thank Richard Hawkes. We thank Hazel Sive, Laure Bally-Cuif, Michael Brand, and Bernard Thisse for kindly providing zebrafish cDNA constructs.Attached Files
Published - 7293.full.pdf
Supplemental Material - KoesterMovie1.mov
Supplemental Material - KoesterMovie2.mov
Supplemental Material - KoesterMovie3.mov
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Additional details
- PMCID
- PMC6673949
- Eprint ID
- 64453
- Resolver ID
- CaltechAUTHORS:20160212-083833906
- NIH
- Caltech Biological Imaging Center
- Deutsche Forschungsgemeinschaft (DFG)
- 0311889
- Created
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2016-02-19Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field