How Different DNA-Binding Proteins Affect Long-Range Oxidative Damage to DNA
- Creators
- Rajski, Scott R.
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Barton, Jacqueline K.
Abstract
Here the effect on DNA-mediated charge transport of binding by a variety of proteins is examined. DNA assemblies were constructed that contain a tethered rhodium intercalator, as photooxidant, as well as two 5'-GG-3' sites flanking the DNA-binding site for the different proteins. By monitoring the ratio of oxidative damage promoted at the guanine doublet situated distal to the protein-binding site versus that at the proximal site as a function of protein binding, the effects of binding the proteins on DNA-mediated charge transport were determined. Proteins examined included both the wild-type and mutant methyltransferase, M.HhaI, which are base-flipping enzymes, the restriction endonuclease R.PvuII, a TATA-binding protein, which kinks the DNA, and the transcription factor Antennapedia homeodomain protein, which binds DNA through a helix−turn−helix motif. In general, it was observed that yields of long-range oxidative damage correlate with protein-dependent alterations in DNA base stacking. Interactions that disturb the DNA π-stack inhibit DNA charge transport. Alternatively, interactions that promote no helix distortion but, as a result of tight packing, may rigidify the π-stack, serve instead to enhance the ability of the DNA base pairs to serve as a conduit for charge transport. Thus, protein binding to DNA modulates long-range charge transport both negatively and positively, depending upon the specific protein/DNA interactions in play. Long-range DNA charge transport and this modulation by protein binding may be important to consider physiologically.
Additional Information
© 2001 American Chemical Society. Received November 22, 2000; Revised Manuscript Received March 1, 2001. Publication Date (Web): April 12, 2001. Supported by NIH Grant GM49216. We thank our close co-workers Sanjay Kumar and Richard J. Roberts of New England Biolabs (Beverly, MA) for samples of wild-type and M.HhaI Q237W. We also thank Professor Carl S. Parker (Caltech) for the generous gift of ANTP-binding domain peptide and Professors John Horton and Xiaodong Cheng of Emory University for samples of R.PvuII.Additional details
- Eprint ID
- 64349
- Resolver ID
- CaltechAUTHORS:20160209-152857175
- NIH
- GM49216
- Created
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2016-02-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field