Published March 2016
| Accepted Version + Supplemental Material
Journal Article
Open
Proton-coupled electron hopping in Ru-modified P. aeruginosa azurin
Abstract
We constructed two artificial multiple-step electron transfer (hopping) systems based on Pseudomonas aeruginosa azurin where a tyrosine (YOH) is situated between Ru(2,2′-bipyridine)_2(imidazole)(histidine) and the native copper site: RuH107YOH109 and RuH124-YOH122. We investigated the rates of Cu^I oxidation by flash-quench generated Ru^(III) over a range of conditions that probed the role of proton-coupled oxidation/reduction of YOH in the reaction. Rates of Cu^I oxidation were enhanced over single-step electron transfer by factors between 3 and 80, depending on specific scaffold and buffer conditions.
Additional Information
© 2016 SBIC. Received: 8 December 2015; Accepted: 4 January 2016. First online: 20 January 2016. Topical Issue in Honor of R.J.P. Williams. Research reported in this publication was supported by The National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number R01DK019038 to HBG and JRW. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Additional support was provided by the Arnold and Mabel Beckman Foundation.Attached Files
Accepted Version - nihms753437.pdf
Supplemental Material - 775_2016_1332_MOESM1_ESM.pdf
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775_2016_1332_MOESM1_ESM.pdf
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Additional details
- PMCID
- PMC4775413
- Eprint ID
- 64036
- Resolver ID
- CaltechAUTHORS:20160127-160435518
- R01DK019038
- NIH
- Arnold and Mabel Beckman Foundation
- Created
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2016-01-28Created from EPrint's datestamp field
- Updated
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2022-05-13Created from EPrint's last_modified field