Published July 2004
| public
Journal Article
Expression patterns of four different regulatory genes that function during sea urchin development
Abstract
The spatial and temporal expression patterns of Strongylocentrotus purpuratus genes encoding four different transcription factors, viz. SpFoxb, SpHes, SpKrl, and SpNk1, have been examined, using a recently developed, highly sensitive whole mount in situ hybridization procedure, and quantitative real time PCR. Two of the genes studied, SpHes and SpNk1, are newly isolated. Their expression patterns suggest the existence of previously unknown ectodermal domains. Re-examination of the expression pattern of SpFoxb reveals domains of expression not previously reported for this gene, and we also provide a more detailed, temporal and spatial description of the expression pattern of SpKrl than heretofore available.
Additional Information
© 2004 Elsevier B.V. Received 11 November 2003; received in revised form 14 December 2003; accepted 6 January 2004. Available online 4 March 2004. We greatly thank Dr Veronica Hinman for instruction in molecular phylogenic analysis, critical reading of the manuscript, and correction of English writing, and Dr Lynne Angerer for a cDNA clone for SpKrl. We are very grateful to Drs Andrew Ransick and Paola Oliveri and Ms Carolina B. Livi of this laboratory for allowing us to discuss their unpublished data. We also thank Ms Jane Wyllie for reading of the draft. We greatly appreciate the expert assistance of Ms Miki Yun for sequencing. This study was supported by the Lucille P. Markey Charitable Trust and NIH Grant HD37105 to EHD.Additional details
- Eprint ID
- 63640
- DOI
- 10.1016/j.modgep.2004.01.009
- Resolver ID
- CaltechAUTHORS:20160113-122350584
- Lucille P. Markey Charitable Trust
- HD37105
- NIH
- Created
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2016-01-13Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field