Regulation of spblimp1/krox1a, an alternatively transcribed isoform expressed in midgut and hindgut of the sea urchin gastrula
- Creators
- Livi, Carolina B.
- Davidson, Eric H.
Abstract
The sea urchin regulatory gene Spblimp1/krox produces alternatively transcribed and spliced isoforms, 1a and 1b, which have different temporal and spatial patterns of expression. Here we describe a cis-regulatory module that controls the expression of the 1a splice form in the midgut and hindgut at the beginning of gastrulation. Conserved sequence patches revealed by a comparison of the blimp1/krox locus in Strongylocentrotus purpuratus and Lytechinus variegatus genomes were tested by gene transfer, in association with GFP or CAT reporter genes. An expression construct containing a conserved sequence patch immediately 5′ of exon 1a included the transcription initiation site for blimp1/krox1a. This construct displays specific mid and hindgut expression, indicating that these are the locations of endogenous blimp1/krox1a transcription during the gastrula stage. Its sequence contains binding sites for Brn1/2/4, Otx, and Blimp1/Krox itself, as predicted in a prior regulatory network analysis.
Additional Information
© 2006 Elsevier B.V. Received 22 December 2005; received in revised form 19 April 2006; accepted 28 April 2006. Available online 10 May 2006. We thank Takuya Minokawa for assistance with fusion PCR, and for sequences for CAT fusion primers and templates, and Titus Brown for assistance with the Family Relations analysis. This work was supported by a grant from the National Institute of Child Health and Human Development.Additional details
- Eprint ID
- 63637
- Resolver ID
- CaltechAUTHORS:20160113-122349753
- National Institute of Child Health and Human Development (NICHD)
- Created
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2016-01-13Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field