Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction
Abstract
Polymerase chain reaction (PCR)-based methods have been developed and increasingly used for rapid and sensitive detection of pathogens in water samples to better protect public health. A propidium monoazide (PMA) pretreatment can help to differentiate between viable and dead cells, but the photoactivation of PMA normally requires the use of an energy-consuming halogen light, which is not suitable for off-the-grid applications. Herein, we investigate sunlight as an alternative light source. Our results suggest that sunlight can successfully activate PMA, and the sunlight-activated PMA pretreatment can effectively reduce the amplification of DNA derived from dead cells in PCR assays. Potentially, a sunlight-activated PMA pretreatment unit can be integrated into a lab-on-a-chip PCR device for off-the-grid microbial detection and quantification.
Additional Information
© 2016 American Chemical Society. Received: December 15, 2015; Revised: December 30, 2015; Accepted: January 5, 2016; Publication Date (Web): January 5, 2016. The authors acknowledge the financial support of the Bill and Melinda Gates Foundation (BMGF OPP1111246). The authors declare no competing financial interest.Attached Files
Supplemental Material - ez5b00348_si_001.pdf
Files
Name | Size | Download all |
---|---|---|
md5:f49fc91dcfc03cdff9199052c89cf323
|
621.1 kB | Preview Download |
Additional details
- Eprint ID
- 63542
- DOI
- 10.1021/acs.estlett.5b00348
- Resolver ID
- CaltechAUTHORS:20160111-103924491
- OPP1111246
- Bill and Melinda Gates Foundation
- Created
-
2016-01-13Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field