Published March 2016
| Published + Supplemental Material
Journal Article
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Characterization of Antibody Bipolar Bridging Mediated by the Human Cytomegalovirus Fc receptor gp68
Abstract
The human cytomegalovirus glycoprotein gp68 functions as an Fc receptor for host IgGs and can form antibody bipolar bridging (ABB) complexes in which gp68 binds the Fc region of an antigen-bound IgG. Here we show that gp68-mediated endocytosis transports ABB complexes into endosomes, after which the complex is routed to lysosomes, presumably for degradation. These results suggest gp68 contributes to evasion of IgG-mediated immune responses by mediating destruction of host IgG and viral antigens.
Additional Information
© 2016 American Society for Microbiology. Received 11 November 2015 Accepted 30 December 2015. Accepted manuscript posted online 6 January 2016. Editor: K. Frueh. We thank Homayon Ghiasi for the gD gene, Hartmut Hengel and Elizabeth R. Sprague for the gp68 gene, Alex Farley for the anti-gD gene constructs, the Caltech Protein Expression Center for expression of antibodies used for internalization, Yunji Wu for purified 2G12 IgG, Marta Murphy for help making figures, and members of the Bjorkman lab for critical reading of the manuscript. This work was supported by the National Institutes of Health (grant R01 AI041239 to P.J.B.) and the Irvington Institute Fellowship Program of the Cancer Research Institute (postdoctoral fellowship to B.N.; www.cancerresearch.org/grants-programs/grants-fellowships). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. FUNDING INFORMATION: HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) http://dx.doi.org/10.13039/100000060R01 AI041239 Pamela J. Bjorkman Cancer Research Institute (CRI) http://dx.doi.org/10.13039/100000884 Blaise NdjamenAttached Files
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Additional details
- PMCID
- PMC4810659
- Eprint ID
- 63537
- Resolver ID
- CaltechAUTHORS:20160111-100543517
- NIH
- R01 AI041239
- Cancer Research Institute
- Created
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2016-01-13Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field