Published September 27, 1993
| public
Journal Article
A modified method for obtaining large amounts of high titer polyclonal ascites fluid
Abstract
When limited amounts of antigens are available, it is often difficult to obtain large quantities of polyclonal antisera. Although antisera can be raised in small rodents, yields are usually small. To circumvent these problems, we have designed a modified method for generating polyclonal ascites fluid (AF). Using the appropriate strain of mice and adjuvant, we generated high serum titers by injection of 1–100 μg of protein. Following i.p. injection of compatible sarcoma cells, 13–19 ml of high titer (1:1000–1:20,000), polyclonal ascites fluid were obtained from each mouse. Similar results were obtained using nine different antigens.
Additional Information
© 1993 Elsevier. (Received 29 March 1993; revised received 1 June 1993; accepted 2 June 1993) This work was supported by NSF grant DIR-8712123. We thank Drs. S. Gharib, A. Golden, M. Kennedy, J. Lee, J.H. Ou and C. Parker for providing the antigens used in this study, Dr. A.E. Karu at U.C. Berkeley for advising the use of S/W mice and Dr. Z. Kaprielian and M.-J. Fann for helpful suggestions on the manuscript.Additional details
- Eprint ID
- 63256
- Resolver ID
- CaltechAUTHORS:20151229-161900447
- DIR-8712123
- NSF
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2021-11-10Created from EPrint's last_modified field