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Published March 2016 | Published + Supplemental Material
Journal Article Open

Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi

Abstract

Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism Vibrio harveyi by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of V. harveyi from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.

Additional Information

© 2015 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. This article is Open Access All publication charges for this article have been paid for by the Royal Society of Chemistry. Received 4th September 2015; Accepted 17th November 2015; First published online 23 Nov 2015. We thank Annie Moradian and Roxana Eggleston-Rangel for technical support with LC-MS/MS experiments. This work was supported by the Howard Hughes Medical Institute, National Institutes of Health (NIH) grant 5R01GM065859 and National Science Foundation (NSF) grant MCB-0343821 to BLB, and by NIH Grant R01GM062523 and the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office to DAT. SH and MJS were supported by the Gordon and Betty Moore Foundation, through Grant GBMF775, and the Beckman Institute. The content of the paper does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. This article is part of themed collection: ISACS16: Challenges in Chemical Biology.

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