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Published November 1, 1970 | Published
Journal Article Open

Conformations of the Single-Stranded DNA of Bacteriophage M13

Abstract

At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100°C in 1.0 M NaCl. The ability to assume either conformation appears to be a function of the nucleic acid alone. Whether or not these different conformations are of biological significance is still unknown.

Additional Information

© 1970 National Academy of Sciences. Communicated by Norman Davidson, August 28, 1970. Supported by NSF grant GB 18074, University of California Cancer Research Funds, and an NSF predoctoral traineeship to A.B.F.

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Created:
August 19, 2023
Modified:
October 25, 2023