TRP channel mediated neuronal activation and ablation in freely behaving zebrafish
Abstract
The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.
Additional Information
© 2015 Nature Publishing Group. Received 03 September 2015; Accepted 09 November 2015; Published online 14 December 2015. We thank A. Chen, A. Cruz, A. Dominguez, J. Engle, S. Khan, K. Molinder, B. Niles, V. Sapin and L. Wang for technical assistance. This work was supported by grants from the US National Institutes of Health (F31-NS07842A to S.C.; F32-NS083099 to K.L.M.; R01-NS26539 and DP1-OD006411 to J.R.F.; R00-NS060996, R01-NS070911 and R01-DA031367 to D.A.P.) and the Mallinckrodt Foundation, the Rita Allen Foundation and the Brain and Behavior Research Foundation (D.A.P.). Author Contributions: D.A.P. conceived of and supervised the project. S.C. and C.N.C. designed, performed, and analyzed all experiments, except physiology experiments, which were designed, performed and analyzed by K.L.M. and J.R.F. All authors collaborated to write the manuscript. The authors declare no competing financial interests.Attached Files
Accepted Version - nihms739996.pdf
Supplemental Material - nmeth.3691-S1.pdf
Supplemental Material - nmeth.3691-S2.zip
Supplemental Material - nmeth.3691-SF1.jpg
Supplemental Material - nmeth.3691-SF10.jpg
Supplemental Material - nmeth.3691-SF2.jpg
Supplemental Material - nmeth.3691-SF3.jpg
Supplemental Material - nmeth.3691-SF4.jpg
Supplemental Material - nmeth.3691-SF5.jpg
Supplemental Material - nmeth.3691-SF6.jpg
Supplemental Material - nmeth.3691-SF7.jpg
Supplemental Material - nmeth.3691-SF8.jpg
Supplemental Material - nmeth.3691-SF9.jpg
Supplemental Material - nmeth.3691-sv1.mp4
Supplemental Material - nmeth.3691-sv2.mp4
Supplemental Material - nmeth.3691-sv3.mp4
Supplemental Material - nmeth.3691-sv4.mp4
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Additional details
- PMCID
- PMC4851460
- Eprint ID
- 61752
- Resolver ID
- CaltechAUTHORS:20151030-151749046
- NIH
- F31-NS07842A
- NIH
- F32-NS083099
- NIH
- R01-NS26539
- NIH
- DP1-OD006411
- NIH
- R00-NS060996
- NIH
- R01-NS070911
- NIH
- R01-DA031367
- Mallinckrodt Foundation
- Rita Allen Foundation
- Brain and Behavior Research Foundation
- Created
-
2016-01-05Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field