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Published December 2015 | Published + Supplemental Material
Journal Article Open

Identification of Genes Uniquely Expressed in the Germ Line Tissues of the Jewel Wasp Nasonia vitripennis

Abstract

The jewel wasp Nasonia vitripennis is a rising model organism for the study of haplo-diploid reproduction characteristic of hymenopteran insects, which include all wasps, bees and ants. We performed transcriptional profiling of the ovary, the female soma, and the male soma of N. vitripennis in order to complement a previously existing transcriptome of the wasp testis. These data were deposited into an open access genome browser for visualization of transcripts relative to their gene models. We used these data to identify the assemblies of genes uniquely expressed in the germ line tissues. We found that one hundred and fifty six protein-coding genes are exclusively expressed in the wasp testis compared to only twenty-two in the ovary. Of the testis-specific genes, eight are candidates for male-specific DNA packaging proteins known as protamines. We found very similar expression patterns of centrosome associated genes in the testis and ovary, arguing that de novo centrosome formation, a key process for development of unfertilized eggs into males, likely does not rely on large scale transcriptional differences between these tissues. In contrast, a number of meiosis-related genes show a bias toward testis-specific expression, despite the lack of true meiosis in N. vitripennis males. These patterns may reflect an unexpected complexity of male gamete production in the haploid males of this organism. Broadly these data add to the growing number of genomic and genetic tools available in N. vitripennis for addressing important biological questions in this rising insect model organism.

Additional Information

Copyright © 2015 Ferree et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Received August 17, 2015. Accepted September 29, 2015. Early Online October 13, 2015. We thank Igor Antoscheckin for input on this manuscript. We also thank Tim Megraw for advice on centrosome orthologs. This work was supported by an NSF CAREER award (NSF1451839) to Patrick M. Ferree and an NIH K22 grant (5K22AI113060-02) to Omar S. Akbari.

Attached Files

Published - GGG021386_2647..pdf

Supplemental Material - Ferree_TableS1.xlsx

Supplemental Material - Ferree_TableS10.xlsx

Supplemental Material - Ferree_TableS11.xlsx

Supplemental Material - Ferree_TableS12.xlsx

Supplemental Material - Ferree_TableS2.xlsx

Supplemental Material - Ferree_TableS3.xlsx

Supplemental Material - Ferree_TableS4.xlsx

Supplemental Material - Ferree_TableS5.xlsx

Supplemental Material - Ferree_TableS6.xlsx

Supplemental Material - Ferree_TableS7.xlsx

Supplemental Material - Ferree_TableS8.xlsx

Supplemental Material - Ferree_TableS9.xlsx

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