A transgenic quail model that enables dynamic imaging of amniote embryogenesis
Abstract
Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.
Additional Information
© 2015 Published by The Company of Biologists Ltd. Received 4 January 2015; Accepted 5 July 2015; Posted online before print July 24, 2015. We thank Charlie Little and members of the R.L. lab for critically reading the manuscript. Competing interests: The authors declare no competing or financial interests. Author contributions: Conceived and designed the experiments: D.H., B.B. and R.L. Performed the experiments: D.H., B.B., A.W., J.Y., M.F. and R.L. Analyzed the data: D.H., B.B., S.E.F. and R.L. Wrote the paper: D.H., B.B. and R.L. Funding: This work was supported by grants from the National Institutes of Health (NIH) National Institute of General Medical Sciences [R01GM102801-01A], National Center for Research Resources [R21HD047347-01], National Human Genome Research Institute Centers of Excellence in Genomic Science program [P50 HG004071] and National Institute of Dental and Craniofacial Research FaceBase Consortium [U01-DE020063)]. M.F. was supported by the G. Harold & Leila Y. Mathers Charitable Foundation in the laboratory of Dr C. Little, University of Kansas Medical Center. Deposited in PMC for release after 12 months.Attached Files
Published - Development-2015-Huss-2850-9.pdf
Supplemental Material - DEV121392supp.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:4d7f0a54b07c45a9e6eab3d269392ab8
|
1.6 MB | Preview Download |
|
md5:fe65ae5b3f8a141c82bccf653bf7f334
|
6.3 MB | Preview Download |
Additional details
- Alternative title
- Transgenic quail to dynamically image amniote embryogenesis
- PMCID
- PMC4550965
- Eprint ID
- 60187
- Resolver ID
- CaltechAUTHORS:20150911-095030467
- NIH
- R01GM102801-01A
- National Center for Research Resources (NCRR)
- R21HD047347-01
- National Human Genome Research Institute
- P50 HG004071
- National Institute of Dental and Craniofacial Research (NIDCR)
- U01-DE020063
- G. Harold and Leila Y. Mathers Charitable Foundation
- Created
-
2015-09-11Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field