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Published April 8, 1977 | public
Journal Article Metadata-only

Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites

Scheller, Richard H.
Thomas, Terry L.
Lee, Amy S.
Klein, William H.
Niles, Walter D.
Britten, Roy J.
Davidson, Eric H.
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Abstract

Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low C_0t followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.

Additional Information

© 1977 American Association for the Advancement of Science. Received 7 February 1977. T4 polynucleotide kinase, T4 DNA ligase, and Eco RI endonuclease were gifts of P. Green, H. Heyneker, and H. Boyer. Supported by NIH grants HD-05753 and GM-20927 and by NSF grant BMS 75-07359. R.H.S. is supported by an NIH predoctoral training grant; T.L.T. by an NIH postdoctoral fellowship; A.S.L. by fellowship J-289 and W.H.K. by Lievre fellowship J-340, both from the American Cancer Society, California Division.

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October 23, 2023