Published May 8, 1997
| public
Journal Article
Regulation of gene expression by small molecules
Chicago
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Abstract
Small molecules that target specific DNA sequences have the potential to control gene expression. Ligands designed for therapeutic application must bind any predetermined DNA sequence with high affinity and permeate living cells. Synthetic polyamides containing N-methylimidazole and N-methylpyrrole amino acids have an affinity and specificity for DNA comparable to naturally occurring DNA-binding proteins. We report here that an eight-ring polyamide targeted to a specific region of the transcription factor TFIIIA binding site interferes with 5S RNA gene expression in Xenopus kidney cells. Our results indicate that pyrrole-imida-zole polyamides are cell-permeable and can inhibit the transcription of specific genes.
Additional Information
© 1997 Nature Publishing Group. Received 24 January; accepted 3 March 1997. We are grateful to the NIH for support of this work, the NSF for a predoctoral fellowship to J.W.T., and the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B.Additional details
- Eprint ID
- 58316
- Resolver ID
- CaltechAUTHORS:20150617-103153814
- NIH
- NSF Predoctoral Fellowship
- Howard Hughes Medical Institute (HHMI)
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2015-06-17Created from EPrint's datestamp field
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2021-11-10Created from EPrint's last_modified field