Published August 2015
| Accepted Version
Journal Article
Open
Recent advances in engineering microbial rhodopsins for optogenetics
Chicago
An error occurred while generating the citation.
Abstract
Protein engineering of microbial rhodopsins has been successful in generating variants with improved properties for applications in optogenetics. Members of this membrane protein family can act as both actuators and sensors of neuronal activity. Chimeragenesis, structure-guided mutagenesis, and directed evolution have proven effective strategies for tuning absorption wavelength, altering ion specificity and increasing fluorescence. These approaches facilitate the development of useful optogenetic tools and, in some cases, have yielded insights into rhodopsin structure–function relationships.
Additional Information
© 2015 Elsevier Ltd. This work was funded by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office (to F.H.A.) and 1R21MH103824-01 from the National Institutes of Health (to F.H.A.). R.S.M. acknowledges funding from the Shurl and Kay Curci Foundation and the Life Sciences Research Foundation. C.N.B. acknowledges support from the NIMH of the NIH for the NRSA fellowship under Award Number F31MH102913.Attached Files
Accepted Version - nihms-690295.pdf
Files
nihms-690295.pdf
Files
(1.2 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:cd9c553349fdb0c9a014e9c8e3b0d2ad
|
1.2 MB | Preview Download |
Additional details
- PMCID
- PMC4641784
- Eprint ID
- 58223
- DOI
- 10.1016/j.sbi.2015.05.001
- Resolver ID
- CaltechAUTHORS:20150612-152121122
- Army Research Office (ARO)
- W911NF-09-0001
- NIH
- 1R21MH103824-01
- Shurl and Kay Curci Foundation
- Life Sciences Research Foundation
- NIH Predoctoral Fellowship
- F31MH102913
- National Institute of Mental Health (NIMH)
- Created
-
2015-06-13Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field