A Chemoenzymatic Approach toward the Rapid and Sensitive Detection of O-GlcNAc Posttranslational Modifications
Abstract
We report a new chemoenzymatic strategy for the rapid and sensitive detection of O-GlcNAc posttranslational modifications. The approach exploits the ability of an engineered mutant of β-1,4-galactosyltransferase to selectively transfer an unnatural ketone functionality onto O-GlcNAc glycosylated proteins. Once transferred, the ketone moiety serves as a versatile handle for the attachment of biotin, thereby enabling chemiluminescent detection of the modified protein. Importantly, this approach permits the rapid visualization of proteins that are at the limits of detection using traditional methods. Moreover, it bypasses the need for radioactive precursors and captures the glycosylated species without perturbing metabolic pathways. We anticipate that this general chemoenzymatic strategy will have broad application to the study of posttranslational modifications.
Additional Information
© 2003 American Chemical Society. Received September 16, 2003. Publication Date (Web): December 6, 2003. We thank Dr. M. Shahgholi, Dr. P. Snow, H.-C. Tai, and S. Tully for helpful discussions and assistance. This research was supported by an NSF CAREER Award (CHE-0239861) and an Alfred P. Sloan Fellowship.Attached Files
Supplemental Material - ja038545rsi20031110_061332_si.pdf
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Additional details
- Eprint ID
- 57689
- DOI
- 10.1021/ja038545r
- Resolver ID
- CaltechAUTHORS:20150520-091857635
- CHE-0239861
- NSF
- Alfred P. Sloan Foundation
- Created
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2015-05-20Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field