Published 2015
| public
Book Section - Chapter
RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
- Others:
- Nakagawa, Shinichi
- Hirose, Tetsuro
Abstract
RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing.
Additional Information
© 2015 Springer. Online: 5 December 2014.Additional details
- Eprint ID
- 57319
- DOI
- 10.1007/978-1-4939-2253-6_11
- Resolver ID
- CaltechAUTHORS:20150507-091048417
- Created
-
2015-05-07Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1262