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Published November 23, 2000 | public
Journal Article

Resonance Raman Spectroscopy of Met121Glu Azurin

Abstract

The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin have been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. Analysis of the 600-nm absorption band and resulting resonance Raman excitation profiles under both pH conditions indicates that the excited-state distortions in this mutant are smaller than those in the native protein.

Additional Information

© 2000 American Chemical Society. Received: March 2, 2000; In Final Form: September 11, 2000. Work at the University of Alberta was supported by NSERC; work at Caltech was supported by NIH DK19038.

Additional details

Created:
August 19, 2023
Modified:
October 23, 2023