Published October 1969
| public
Journal Article
Secondary isotope effects in dehydration of malic acid by fumarate hydratase
Chicago
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Abstract
The kinetic isotope effects for the dehydration by fumarate hydratase of 2-^3H-1,4-^(14)Cl-/-malic acid and 2,3-^3H-1,4-^(14)C-l-malic acid have been determined and support a carbonium ion mechanism for the enzymatic reaction. The isotope effect on the equilibrium between l-malate and fumarate has also been determined experimentally and agrees closely with that anticipated on the basis of a Bigeleisen treatment.
Additional Information
© 1969 American Chemical Society. We should like to thank the National Institutes of Health of the U.S. Public Health Service (Grant GM-10218) for support of this research and Professor J. D. Roberts for a useful suggestion.Additional details
- Eprint ID
- 57085
- Resolver ID
- CaltechAUTHORS:20150429-094618091
- NIH
- GM-10218
- Created
-
2015-04-29Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field
- Other Numbering System Name
- Caltech Gates and Crellin Laboratories
- Other Numbering System Identifier
- 3796