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Published October 1969 | public
Journal Article

Secondary isotope effects in dehydration of malic acid by fumarate hydratase

Abstract

The kinetic isotope effects for the dehydration by fumarate hydratase of 2-^3H-1,4-^(14)Cl-/-malic acid and 2,3-^3H-1,4-^(14)C-l-malic acid have been determined and support a carbonium ion mechanism for the enzymatic reaction. The isotope effect on the equilibrium between l-malate and fumarate has also been determined experimentally and agrees closely with that anticipated on the basis of a Bigeleisen treatment.

Additional Information

© 1969 American Chemical Society. We should like to thank the National Institutes of Health of the U.S. Public Health Service (Grant GM-10218) for support of this research and Professor J. D. Roberts for a useful suggestion.

Additional details

Created:
August 19, 2023
Modified:
October 23, 2023