Genetic blueprint of the zoonotic pathogen Toxocara canis
Abstract
Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host–parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.
Additional Information
© 2015 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ Received: 16 August 2014; Accepted: 11 December 2014; Published: 04 February 2015. This project was funded by the International Science & Technology Cooperation Program of China (grant no. 2013DFA31840; X.-Q.Z. and R.B.G.), the Australian Research Council (ARC) and the National Health and Medical Research Council (NHMRC) of Australia (R.B.G.); it was also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant (VR0007; R.B.G.) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government. Other support from the Australian Academy of Science, Alexander von Humboldt Foundation and Melbourne Water Corporation is gratefully acknowledged. N.D.Y. is an NHMRC Early Career Research (ECRF) Fellow. We acknowledge the contributions of all the staff members at WormBase (www.wormbase.org). We thank the Howard Hughes Medical Institute (HHMI) and the National Institutes of Health (NIH; P.W.S.). We thank BGI-Shenzhen for providing a commercial service for the construction of RNA-seq and genomic libraries, and for sequencing.Attached Files
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Additional details
- PMCID
- PMC4327413
- Eprint ID
- 56167
- Resolver ID
- CaltechAUTHORS:20150327-092534533
- International Science & Technology Cooperation Program of China
- 2013DFA31840
- Australian Research Council
- National Health and Medical Research Council (NHMRC)
- Victorian Life Sciences Computation Initiative (VLSCI)
- VR0007
- Australian Academy of Science
- Alexander von Humboldt Foundation
- Melbourne Water Corporation
- Howard Hughes Medical Institute (HHMI)
- NIH
- Created
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2015-03-27Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field