Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published June 28, 2006 | Supplemental Material
Journal Article Open

Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin

Abstract

Parkinson's disease is the second most common neurodegenerative disorder and is characterized by the degeneration of dopaminergic neurons in the substantia nigra. Mitochondrial dysfunction has been implicated as an important trigger for Parkinson's disease-like pathogenesis because exposure to environmental mitochondrial toxins leads to Parkinson's disease-like pathology. Recently, multiple genes mediating familial forms of Parkinson's disease have been identified, including PTEN-induced kinase 1 (PINK1 ; PARK6 ) and parkin (PARK2 ), which are also associated with sporadic forms of Parkinson's disease. PINK1 encodes a putative serine/threonine kinase with a mitochondrial targeting sequence. So far, no in vivo studies have been reported for pink1 in any model system. Here we show that removal of Drosophila PINK1 homologue (CG4523; hereafter called pink1) function results in male sterility, apoptotic muscle degeneration, defects in mitochondrial morphology and increased sensitivity to multiple stresses including oxidative stress. Pink1 localizes to mitochondria, and mitochondrial cristae are fragmented in pink1 mutants. Expression of human PINK1 in the Drosophila testes restores male fertility and normal mitochondrial morphology in a portion of pink1 mutants, demonstrating functional conservation between human and Drosophila Pink1. Loss of Drosophila parkin shows phenotypes similar to loss of pink1 function. Notably, overexpression of parkin rescues the male sterility and mitochondrial morphology defects of pink1 mutants, whereas double mutants removing both pink1 and parkin function show muscle phenotypes identical to those observed in either mutant alone. These observations suggest that pink1 and parkin function, at least in part, in the same pathway, with pink1 functioning upstream of parkin. The role of the pink1–parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease pathogenesis.

Additional Information

© Nature Publishing Group. Received 15 February; accepted 7 April 2006; Published online 30 April 2006. We thank G. Mardon and L. Pallanck for parkin cDNA and mutant flies; A. Simon, D. Walker, X. Zhan and A. Kiger for technical advice; L. Zipursky, L. Toro and D. Krantz for access to equipment and space; Guo laboratory members for discussions; and the EM core facilities at UCLA Brain Research Institute and at Caltech. We are indebted to R. Young in Seymour Benzer's laboratory for assistance with EM, and F. Laski for his phase contrast microscope. This work was supported by a National Institute of Health (NIH) grant to B.A.H. and an Alfred P. Sloan Foundation Fellowship in Neuroscience and a NIH grant to M.G. Author Contributions I.E.C., M.W.D., C.J. and J.H.C. in the Guo laboratory conceived and performed the experiments. J.R.H. and B.A.H. in the Hay laboratory assisted with experiments involving TEM in testes and with TUNEL staining; J.H.S. and S.J.Y. provided crucial reagents; and M.G. conceived and performed experiments, supervised the work, and wrote the manuscript with helpful comments from B.A.H. and authors from the Guo laboratory.

Attached Files

Supplemental Material - nature04779-s1.jpg

Supplemental Material - nature04779-s2.jpg

Supplemental Material - nature04779-s3.jpg

Supplemental Material - nature04779-s4.doc

Files

nature04779-s1.jpg
Files (1.0 MB)
Name Size Download all
md5:f1a0a8fee118b3e93b52cc15634496f9
530.1 kB Preview Download
md5:ce7adb767d99a3d859ab2bc71297fd90
39.9 kB Download
md5:cef8b2749b64234b7e01362ed7e9f91c
261.5 kB Preview Download
md5:53b2ca0c01b358819ec2b51d6d88a142
174.0 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023