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Published March 8, 2007 | public
Journal Article

Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells

Lim, Mi Hee

Abstract

A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide (NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield ~20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of ~26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy.

Additional Information

© 2007 Nature Publishing Group. Published online 8 March 2007. I gratefully acknowledge Professor Stephen J. Lippard for his support, helpful suggestions and inspiring discussions. This work was supported by a grant from the National Science Foundation (to S.J.L.). I further wish to thank the Martin Family Society at MIT for a fellowship.

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023