Published March 8, 2007
| public
Journal Article
Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells
- Creators
- Lim, Mi Hee
Abstract
A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide (NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield ~20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of ~26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy.
Additional Information
© 2007 Nature Publishing Group. Published online 8 March 2007. I gratefully acknowledge Professor Stephen J. Lippard for his support, helpful suggestions and inspiring discussions. This work was supported by a grant from the National Science Foundation (to S.J.L.). I further wish to thank the Martin Family Society at MIT for a fellowship.Additional details
- Eprint ID
- 55859
- DOI
- 10.1038/nprot.2007.43
- Resolver ID
- CaltechAUTHORS:20150317-132252147
- NSF
- Massachusetts Institute of Technology (MIT)
- Created
-
2015-03-17Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field