Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published January 25, 1976 | Published
Journal Article Open

Linear Structure of the Oligosaccharide Chains in α_1-Protease Inhibitor Isolated from Human Plasma

Abstract

Two glycopeptides present in equal amounts were isolated from a pronase digest of alpha1-protease inhibitor of human plasma by gel filtration on Sephadex G-50 and chromatography on DEAE-cellulose. The carbohydrate side chains in both glycopeptides are linked through asparaginyl residues. The glycopeptides were digested sequentially with specific glycosidases; and after each step, the released sugars as well as the composition of the residual peptides were determined. The linear structures of these glycopeptides deduced from these data are shown below. Based on the total carbohydrate content of the intact protein and with these structural data, it is postulated that 4 oligosaccharide units are attached to 1 molecule of the protein; 2 of these were represented as in Equation 1, the other 2 as in Equation 2.

Additional Information

© 1976 American Society for Biochemistry and Molecular Biology. Received for publication, December 9, 1974 and in revised form, May 1, 1975. This work was supported in part by Research Grants HL 16166 from the National Institutes of Health (to S. K. C.) and National Science Foundation Grant GB 43571 (to Y.T. L.).

Attached Files

Published - 4_chan_1976.pdf

Files

4_chan_1976.pdf
Files (634.0 kB)
Name Size Download all
md5:485bcfe23a96036280c5c3109357b18f
634.0 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023