Published September 14, 1984
| public
Journal Article
Echinomycin Binding Sites on DNA
- Creators
- Van Dyke, Michael M.
-
Dervan, Peter B.
Abstract
The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A ^(32)P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA. Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).
Additional Information
© 1984 American Association for the Advancement of Science. 8 May 1984; accepted 28 June 1984. Supported by the American Cancer Society research grant number NP-428, an unrestricted grant from Burroughs Wellcome Co. (P.B.D.), and a National Research Service Award (GM-07616) (to M.W.V.). We thank Dr. A. Rich for informing us of his results before publication. Address correspondence to P .B.D.Additional details
- Eprint ID
- 54542
- Resolver ID
- CaltechAUTHORS:20150209-103133277
- NP-428
- American Cancer Society
- Burroughs Wellcome
- GM07616
- NIH
- Created
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2015-02-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field