Molecular Analysis of the Hotspot of Recombination in the Murine Major Histocompatibility Complex
Abstract
Biological and serological assays have been used to define four subregions for the I region of the major histocompatibility complex (MHC) in the order I-A, I-B, I-J, and I-E. The I-J subregion presumably encodes the I-J polypeptide of the elusive T-cell suppressor factors. Restriction enzyme site polymorphisms and DNA sequence analyses of the I region from four recombinant mouse strains were used to localize the putative I-B and I-J subregions to a 1.0-kilobase (kb) region within the E_β gene. Sequencing this region from E_β clones derived from the two mouse strains: B10.A(3R), I-J^b and B10.A(5R), I-J^k initially used to define the I-J subregion revealed that these regions are identical, hence the distinct I-Jb and I-J^k molecules cannot be encoded by this DNA. In addition, the DNA sequence data also refute the earlier mapping of the I-B subregion. Analysis of the DNA sequences of three parental and four I region recombinants reveals that the recombinant events in three of the recombinant strains occurred within a 1-k^b region of DNA, supporting the proposition that a hotspot for recombination exists in the I region. The only striking feature of this hotspot is a tetramer repeat (AGGC)n that shows 80 percent homology to the minisatellite sequence which may facilitate recombination in human chromosomes.
Additional Information
© 1986 American Association for the Advancement of Science. 2 April 1986; accepted 20 August 1986. We thank C. Graham, L. Tomich, and S. Horvath for synthesis of oligonucleotides; C. Prussin for technical assistance; D. Maloney and S. Davis for preparation of sequencing gels; M. DeBruyn for media; C. Katz and C. Elkins for typing; B. Jones for assistance in preparing figures; T. Hunkapiller, B. Arden, R. Barth, M. Kronenberg, E. Meyerowitz, H. Passmore, N. Shastri, G. Siu, L. Smith, and B. Wold for critical discussions during the course of this work and comments on the manuscript. Supported by a postdoctoral fellowship from the Arthritis Foundation (J.A.K.).Additional details
- Eprint ID
- 54530
- DOI
- 10.1126/science.3018929
- Resolver ID
- CaltechAUTHORS:20150209-095005901
- Arthritis Foundation
- Created
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2015-02-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field