Participation of the protein G_o in multiple aspects of behavior in C. elegans
Abstract
The goa-1 gene encoding the alpha subunit of the heterotrimeric guanosine triphosphatebinding protein (G protein) G_o. from Caenorhabditis elegans is expressed in most neurons, and in the muscles involved in egg laying and male mating. Reduction-of-function mutations in goa-1 caused a variety of behavioral defects including hyperactive movement, premature egg laying, and male impotence. Expression of the activated G_o alpha subunit (Gα_o) in transgenic nematodes resulted in lethargic movement, delayed egg laying, and reduced mating efficiency. Induced expression of activated Gα_o in adults was sufficient to cause these phenotypes, indicating that Gα_o mediates behavior through its role in neuronal function and the functioning of specialized muscles.
Additional Information
© 1995 American Association for the Advancement of Science. 8 October 1994; accepted 19 January 1995. We thank S. Gharib for technical assistance and members of our laboratories for critical reading of the manuscript. Some strains were from the Caenorhabditis Genetics Center. Supported by a grant from the Human Frontier Science Program to M.I.S., R.H.A.P., and P.W.S., and by the Howard Hughes Medical Institute of which P.W.S. is an investigator.Additional details
- Alternative title
- Participation of the protein Go in multiple aspects of behavior in C. elegans
- Eprint ID
- 54065
- DOI
- 10.1126/science.7886455
- Resolver ID
- CaltechAUTHORS:20150126-093856927
- Human Frontier Science Program
- Howard Hughes Medical Institute (HHMI)
- Created
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2015-01-26Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field