Published August 18, 1989
| public
Journal Article
Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation
Abstract
Oligonucleotides that bind to duplex DNA in a sequence-specific manner by triple helix formation offer an approach to the experimental manipulation of sequence-specific protein binding. Micromolar concentrations of pyrimidine oligodeoxyribonucleotides are shown to block recognition of double helical DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor at a homopurine target site. Inhibition is sequence-specific. Oligonucleotides containing 5-methylcytosine provide substantially more efficient inhibition than oligonucleotides containing cytosine. The results have implications for gene-specific repression by oligonucleotides or their analogs.
Additional Information
© 1989 American Association for the Advancement of Science. 21 March 1989; Accepted 5 July 1989. We thank R. Tjian for pSpl-516C, and we also thank members of the Dervan and Wold groups for helpful discussions. Supported by grants from the Caltech Beckman Institute and the NIH (B.W. and P.B.D.), by a Gosney Fellowship, and an American Cancer Society Fellowship to L.J.M.Additional details
- Eprint ID
- 53822
- Resolver ID
- CaltechAUTHORS:20150116-103837502
- Caltech Beckman Institute
- NIH
- Gosney Fellowship
- American Cancer Society
- Created
-
2015-01-16Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field