cDNA clone encoding Drosophila transcription factor TFIID
Abstract
Proper initiation of transcription by RNA polymerase II requires the TATA-consensus-binding transcription factor TFIID. A cDNA clone encoding the Drosophila TFIID protein has been isolated and characterized. The deduced amino acid sequence reveals an open reading frame of 353 residues. The carboxyl-terminal 180 amino acids are approximately 80% identical to yeast TFIID and 88% identical to human TFIID. The amino-terminal portions of the yeast and Drosophila TFIID proteins lack appreciable homology, whereas the Drosophila and human amino termini appear qualitatively similar. In addition, the amino-terminal region of the Drosophila TFIID contains several sequence motifs that are found in other Drosophila proteins which appear to regulate transcription.
Additional Information
© 1990 National Academy of Sciences. Communicated by John Abelson, August 27, 1990 (received for review June 29, 1990). We thank Alexander Hoffman (Rockefeller University) for communicating unpublished sequence data. M.L.M. was a recipient of a National Institutes of Health postdoctoral fellowship (F32 GM12244) and a fellowship from the American Cancer Society, California Division (S-38-88). C.T.I. was a recipient of a postdoctoral fellowship from the American Cancer Society, National Division (PF-03083). This research was supported by National Institutes of Health Grant GM29430 to C.S.P. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - PNAS-1990-Muhich-9148-52.pdf
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Additional details
- PMCID
- PMC55121
- Eprint ID
- 53615
- Resolver ID
- CaltechAUTHORS:20150113-092334309
- NIH Postdoctoral Fellowship
- F32 GM12244
- American Cancer Society
- S-38-88
- American Cancer Society
- PF-03083
- NIH
- GM29430
- Created
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2015-01-13Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field