Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published February 1999 | Published
Journal Article Open

Structures of Ruthenium-modified Pseudomonas aeruginosa Azurin and [Ru(2,2'-bipyridine)_2(imidazole)_2)]SO_4•10H_2O

Abstract

The crystal structure of Ru(2,2'-bipyridine)_2(imidazole)(His83)azurin (RuAz) has been determined to 2.3 Å ¬resolution by X-ray crystallography. The spectroscopic and thermodynamic properties of both the native protein and [Ru(2,2'-bipyridine)_2(imidazole)_2]^(2+) are maintained in the modified protein. Dark-green RuAz crystals grown from PEG 4000, LiNO_3, CuCl_2 and Tris buffer are monoclinic, belong to the space group C2 and have cell parameters a = 100.6, b = 35.4, c = 74.7 Å and β = 106.5°. In addition, [Ru(2,2'-bipyridine)_2(imidazole)_2]SO_4•10H_2O was synthesized, crystallized and structurally characterized by X-ray crystallography. Red-brown crystals of this complex are monoclinic, space group P2_1/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) Å, β = 108.65 (2)°. Stereochemical parameters for the refinement of Ru(2,2'-bipyridine)_2(imidazole)(His83) were taken from the atomic coordinates of [Ru(2,2'-bipyridine)_2(imidazole)_2]^(2+). The structure of RuAz confirms that His83 is the only site of chemical modification and that the native azurin structure is not perturbed significantly by the ruthenium label.

Additional Information

© 1999 International Union of Crystallography. Received 29 January 1998; Accepted 27 July 1998. We thank Michael Harrington, Jack Mizoguchi, Larry Henling and Jack Richards for helpful discussions. This work was supported by NIH (DK19038 to HBG; GM45162 to DCR).

Attached Files

Published - 108_faham_1998.pdf

Files

108_faham_1998.pdf
Files (563.9 kB)
Name Size Download all
md5:da18bf39bc94c31dcb4865513bde43ab
563.9 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023