Location and Chemical Synthesis of a Pre-S Gene Coded Immunodominant Epitope of Hepatitis B Virus
Abstract
Immunodominant, disulfide-bond independent epitopes recognized by human antibodies to hepatitis B virus (HBV) are located within the 55-residue amino terminal portion (coded for by the pre-S region of HBV DNA) of minor HBV envelope components larger than the major protein constituents encoded by the S gene. A peptide having the sequence of the first 26 amino acids from the amino terminal methionine was synthesized and elicited antibodies (at dilutions of greater than or equal to 1 to 10^5) to the HBV envelope. These antibodies can be utilized for diagnostic tests. The immunogenicity of the peptide was substantially increased by covalent attachment to liposomes. The disulfide bond-independent determinants on sequences coded for by the pre-S gene may be more easily mimicked by peptide analogs than "conformational" determinants on the S-gene product.
Additional Information
© 1984 American Association for the Advancement of Science. Received 6 February 1984; accepted 15 March 1984. We thank T. Huima, P. Sproul, and W. Lee for assistance. Partially supported by National Heart, Lung and Blood Institute grant 09011.Additional details
- Eprint ID
- 53293
- DOI
- 10.1126/science.6200931
- Resolver ID
- CaltechAUTHORS:20150107-134728246
- 09011
- National Heart, Lung and Blood Institute
- Created
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2015-01-08Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field