Role of a raf Proto-Oncogene during Caenorhabditis elegans Vulval Development
- Creators
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Sternberg, Paul W.
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Golden, Andy
- Han, Min
Abstract
During Caenorhabditise legans vulval induction, multipotent precursors respond to an inductive signal by generating vulval cells as opposed to non-specialized epidermal cells. Both classical and 'reverse' genetic approaches have revealed that a cascade of nematode homologues of mammalian proto-oncogenes is necessary for induction of the vulva. The inductive signal is a growth factor encoded by the lin-3 gene and its candidate receptor is a tyrosine kinase encoded by the let-23 gene. let-23 acts via a Ras protein encoded by the let-60 gene. A nematode homologue of mammalian raf family protein serine/threonine kinases has been cloned and found to be encoded by the lin-45 gene. Dominant negative lin-45 raf mutants prevent vulval induction. A recessive lin-45 raf mutation prevents the excessive vulval differentiation caused by activated ras, indicating that raf might act downstream of ras during vulval induction.
Additional Information
© 1993 The Royal Society and the authors. We are grateful to Phoebe Tzou for technical assistance. This research was supported by the USPHS grants to P.W.S. and M.H., and by the Howard Hughes Medical Institute. P.W.S. is investigator of the Howard Hughes Medical Institute. A.G. is USPHS postdoctoral fellow. M.H. is a Lucille P. Markey Scholar. Strains were provided by the Caenorhabditis Genetics Center.Additional details
- Eprint ID
- 53265
- Resolver ID
- CaltechAUTHORS:20150107-102655698
- USPHS
- Howard Hughes Medical Institute (HHMI)
- Lucille P. Markey Charitable Trust
- Created
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2015-01-07Created from EPrint's datestamp field
- Updated
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2020-03-09Created from EPrint's last_modified field