Simultaneous generation of many RNA-seq libraries in a single reaction
Abstract
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
Additional Information
© 2015 Macmillan Publishers Limited. Received 27 October 2014. Accepted 08 February 2015. Published online 02 March 2015. Accession codes. NCBI Sequence Read Archive: SRP051252. We thank all members of Lander and Guttman labs for their help, especially J.M. Engreitz, P. McDonel and K. Sirokman; L. Gaffney for assistance with figures; and C. Nusbaum for helpful suggestions on the manuscript. We thank I. Antoshechkin and the Millard and Muriel Jacobs Genetics and Genomics Laboratory at Caltech. This work was supported by a US National Institutes of Health (NIH) Director's Early Independence Award (DP5OD012190 to M. Guttman), funds from the Broad Institute of MIT and Harvard and the California Institute of Technology (M. Guttman) and funds from the US National Institute of Allergy and Infectious Diseases, NIH, Department of Health and Human Services, under contract no. HHSN272200900018C.Attached Files
Accepted Version - nihms747505.pdf
Supplemental Material - nmeth.3313-S1.pdf
Supplemental Material - nmeth.3313-S2.xlsx
Supplemental Material - nmeth.3313-S3.xlsx
Supplemental Material - nmeth.3313-S4.xlsx
Supplemental Material - nmeth.3313-S6.xlsx
Supplemental Material - nmeth.3313-S7.xlsx
Supplemental Material - nmeth.3313-S8.xlsx
Supplemental Material - nmeth.3313-SF1.jpg
Supplemental Material - nmeth.3313-SF2.jpg
Supplemental Material - nmeth.3313-SF3.jpg
Supplemental Material - nmeth.3313-SF4.jpg
Supplemental Material - nmeth.3313-SF5.jpg
Supplemental Material - nmeth.3313-SF6.jpg
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Additional details
- PMCID
- PMC4712044
- Eprint ID
- 53208
- DOI
- 10.1038/nmeth.3313
- Resolver ID
- CaltechAUTHORS:20150106-114459354
- NIH
- DP50D012190
- Broad Institute of MIT and Harvard
- Caltech
- National Institute of Allergy and Infectious Diseases
- Department of Health and Human Services
- HHSN272200900018C
- Created
-
2015-03-06Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field