Published August 26, 1988
| public
Journal Article
A Ligase-Mediated Gene Detection Technique
Abstract
An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
Additional Information
© 1988 American Association for the Advancement of Science. Received 11 April 1988; accepted 23 June 1988. The authors acknowledge a stipend from the Knut and Alice Wallenberg Foundation to U.L. and support from NSF grant BNS 87 14486, Defense Advanced Research Projects Agency grant N00014-86K-0755, Upjohn Company, and Applied Biosystems, Inc. The oligonucleotides were synthesized by S. J. Horvath and the fluorescence data were analyzed by C. Dodd. R. K. Saiki provided plasmids and samples of genomic DNA obtained from cell lines. J. Korenberg and K. Tanaka made available blood samples from sickle cells patients. The Nhydroxysuccinimide ester of carboxy-2',7'-dimethoxy- 4',5'-dichlorofluorescein was provided by M. W. Hunkapiller. We acknowledge discussions with B. Korber, B. Popko, A. Kamb, N. Lan, L. Smith, R. Barth, V. A. McKusick, J. Richards, and M. Simon.Additional details
- Eprint ID
- 53120
- DOI
- 10.1126/science.3413476
- Resolver ID
- CaltechAUTHORS:20141222-153953460
- Knut and Alice Wallenberg Foundation
- BNS 87 14486
- NSF
- N00014-86K-0755
- Office of Naval Research (ONR)
- Upjohn Company
- Applied Biosystems, Inc.
- Defense Advanced Research Projects Agency (DARPA)
- Created
-
2014-12-23Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field